Anianabacter salinae gen. nov., sp. nov. ASV31T, a Facultative Alkaliphilic and Extremely Halotolerant Bacterium Isolated from Brine of a Millennial Continental Saltern

During a prokaryotic diversity study in Añana Salt Valley, new Rhodobacteraceae member, designated ASV31T, was isolated from Santa Engracia spring water. It extremely halotolerant, tolerating up to 23% NaCl, and facultatively alkaliphilic, growing at pH 6.5–9.5 (optimum 7.0–9.5). The isolate Gram-negative, rod-shaped, aerobic non-motile bacterium that formed beige-to-pink colonies on marine agar. According 16S rRNA gene-based phylogenetic analysis, strain ASV31T forms distinct branch of the family Rhodobacteraceae, with Thioclava pacifica DSM 10166T being its closest type (95.3%). This confirmed phylogenomic tree values ANI (73.9%), dDDH (19.3%), AAI (63.5%) POCP (56.0%), which were below genus/species level boundary. Additionally, an ability degrade aromatic compounds biosynthesise secondary metabolites suggested by genome ASV31T. Distinguishing fatty acid profiles polar lipid content also observed. size 3.6 Mbp, DNA G+C 65.7%. Based data obtained, it considered (=CECT 30309T = LMG 32242T) represents species genus for name Anianabacter salinae gen. nov., sp. nov. is proposed.